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Responses of respiration in the light to warming in field-grown trees: a comparison of the thermal sensitivity of the Kok and Laisk methods.

Authors :
Way DA
Aspinwall MJ
Drake JE
Crous KY
Campany CE
Ghannoum O
Tissue DT
Tjoelker MG
Source :
The New phytologist [New Phytol] 2019 Apr; Vol. 222 (1), pp. 132-143. Date of Electronic Publication: 2018 Nov 30.
Publication Year :
2019

Abstract

The Kok and Laisk techniques can both be used to estimate light respiration R <subscript>light</subscript> . We investigated whether responses of R <subscript>light</subscript> to short- and long-term changes in leaf temperature depend on the technique used to estimate R <subscript>light</subscript> . We grew Eucalyptus tereticornis in whole-tree chambers under ambient temperature (AT) or AT + 3°C (elevated temperature, ET). We assessed dark respiration R <subscript>dark</subscript> and light respiration with the Kok (R <subscript>Kok</subscript> ) and Laisk (R <subscript>Laisk</subscript> ) methods at four temperatures to determine the degree of light suppression of respiration using both methods in AT and ET trees. The ET treatment had little impact on R <subscript>dark</subscript> , R <subscript>Kok</subscript> or R <subscript>Laisk</subscript> . Although the thermal sensitivities of R <subscript>Kok</subscript> or R <subscript>Laisk</subscript> were similar, R <subscript>Kok</subscript> was higher than R <subscript>Laisk</subscript> . We found negative values of R <subscript>Laisk</subscript> at the lowest measurement temperatures, indicating positive net CO <subscript>2</subscript> uptake, which we propose may be related to phosphoenolpyruvate carboxylase activity. Light suppression of R <subscript>dark</subscript> decreased with increasing leaf temperature, but the degree of suppression depended on the method used. The Kok and Laisk methods do not generate the same estimates of R <subscript>light</subscript> or light suppression of R <subscript>dark</subscript> between 20 and 35°C. Negative rates of R <subscript>Laisk</subscript> imply that this method may become less reliable at low temperatures.<br /> (© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.)

Details

Language :
English
ISSN :
1469-8137
Volume :
222
Issue :
1
Database :
MEDLINE
Journal :
The New phytologist
Publication Type :
Academic Journal
Accession number :
30372524
Full Text :
https://doi.org/10.1111/nph.15566