One, Two, Three: A Bioorthogonal Triple Labelling Strategy for Studying the Dynamics of Plant Cell Wall Formation In Vivo.

Authors :: Simon C
Lion C
Spriet C
Baldacci-Cresp F
Hawkins S
Biot C
Source :: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2018 Dec 17; Vol. 57 (51), pp. 16665-16671. Date of Electronic Publication: 2018 Nov 21.
Publication Year :: 2018
Abstract: Reported herein is an in vivo triple labelling strategy to monitor the formation of plant cell walls. Based on a combination of copper-catalysed alkyne-azide cycloaddition (CuAAC), strain-promoted azide-alkyne cycloaddition (SPAAC), and Diels-Alder reaction with inverse electronic demand (DAR <subscript>inv</subscript> ), this methodology can be applied to various plant species of interest in research. It allowed detection of the differential incorporation of alkynyl-, azido-, and methylcyclopropenyl-tagged reporters of the three main monolignols into de novo biosynthesized lignin in different tissues, cell types, or cell wall layers. In addition, this triple labelling was implemented with different classes of chemical reporters, using two monolignol reporters in conjunction with alkynylfucose to simultaneously monitor the biosynthesis of lignin and non-cellulosic polysaccharides. This allowed observation of their deposition occurring contemporaneously in the same cell wall.<br /> (© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Subjects :: Cell Wall chemistry
Lignin chemistry
Molecular Structure
Polysaccharides chemistry
Cell Wall metabolism
Lignin biosynthesis
Plants metabolism
Polysaccharides biosynthesis

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