Back to Search
Start Over
Characterization of myofiber-type-specific molecules using mass spectrometry imaging.
- Source :
-
Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2019 Jan 30; Vol. 33 (2), pp. 185-192. - Publication Year :
- 2019
-
Abstract
- Rationale: In skeletal muscles, there are four myofiber types, Types I, IIa, IIx, and IIb, which show different contraction characteristics and have different metabolic statuses. To understand muscle function, it is necessary to analyze myofiber-specific metabolic changes. However, these fibers are heterogeneous and are hard to discriminate by conventional analyses using tissue extracts. In this study, we found myofiber-specific molecules and molecular markers of other cells such as smooth muscle cells, fat cells, and motor neurons, and visualized them within muscle sections.<br />Methods: We used three different muscle tissues, namely extensor digitorum longus, soleus, and gastrocnemius tissues, from ICR mice. After the muscles had been harvested, cross-sections were prepared using a cryostat and analyzed using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI), and conventional immunofluorescence imaging.<br />Results: By comparing the MALDI MSI results with the immunofluorescence imaging results, we were able to identify each fiber and cell-specific ion. It was especially important that we could find Type IIa and IIb specific ions, because these were difficult to distinguish.<br />Conclusions: Through MSI analyses, we performed a comprehensive survey to identify cell- and myofiber-specific molecular markers. In conclusion, we assigned muscle fiber Type I, IIa, and IIb-specific molecular ions at m/z 856.6, 872.6, and 683.8, respectively. These molecular markers might be useful for verifying changes that occur due to exercise and/or disease.<br /> (© 2018 John Wiley & Sons, Ltd.)
- Subjects :
- Animals
Biomarkers metabolism
Chromatography, Thin Layer
Diglycerides analysis
Diglycerides metabolism
Image Processing, Computer-Assisted
Lipids analysis
Male
Mice, Inbred ICR
Muscle Fibers, Skeletal metabolism
Muscle, Skeletal chemistry
Muscle, Skeletal metabolism
Muscle, Smooth chemistry
Muscle, Smooth metabolism
Tandem Mass Spectrometry
Biomarkers analysis
Muscle Fibers, Skeletal chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
Subjects
Details
- Language :
- English
- ISSN :
- 1097-0231
- Volume :
- 33
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Rapid communications in mass spectrometry : RCM
- Publication Type :
- Academic Journal
- Accession number :
- 30367536
- Full Text :
- https://doi.org/10.1002/rcm.8319