Back to Search Start Over

[Substrate specificity of restriction endonuclease VspI].

Authors :
Degtiarev SKh
Repin VE
Rechkunova NI
Chizhikov VE
Malygin EG
Source :
Bioorganicheskaia khimiia [Bioorg Khim] 1987 Mar; Vol. 13 (3), pp. 420-1.
Publication Year :
1987

Abstract

The recognition sequence and cleavage point of restriction endonuclease VspI have been determined as 5'-AT decreases TAAT. This enzyme is not isoschizomer of any known restriction endonucleases. DNA pBR322 contains a single VspI recognition sequence in position 3539. Therefore this enzyme may be used for cloning DNA in the VspI site in AmpR-gene of pBR322.

Subjects

Subjects :
Base Sequence
DNA Restriction Enzymes isolation & purification
Substrate Specificity
DNA Restriction Enzymes genetics
Deoxyribonucleases, Type II Site-Specific
Vibrio enzymology

Details

Language :
Russian
ISSN :
0132-3423
Volume :
13
Issue :
3
Database :
MEDLINE
Journal :
Bioorganicheskaia khimiia
Publication Type :
Academic Journal
Accession number :
3036169

Tools

Authors Abstract Subjects Details