Back to Search
Start Over
Ex-Vivo Signal Transduction Studies in Chronic Lymphocytic Leukemia.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1881, pp. 1-17. - Publication Year :
- 2019
-
Abstract
- Microenvironmental signaling is pivotal to chronic lymphocytic leukemia (CLL) pathology; therefore understanding how to investigate this pathway by both protein and chemical methods is crucial if we are to investigate and correlate biological changes with therapeutic responses in patients. Herein, we describe the use of western blotting also referred to as immunoblotting as a method that can semiquantitatively evaluate changes in protein expression following receptor engagement; this includes B cell receptor (BCR) signaling following stimulation with anti-IgM (Blunt et al. Clin Cancer Res 23(9):2313-2324, 2017). It is important to note that immunoblotting should always be combined with other quantitative methods such as flow cytometry to confirm activation of these signaling pathways (Aguilar-Hernandez et al. Blood 127(24):3015-3025, 2016).
- Subjects :
- B-Lymphocytes metabolism
Blotting, Western instrumentation
Electrophoresis, Polyacrylamide Gel instrumentation
Electrophoresis, Polyacrylamide Gel methods
Flow Cytometry instrumentation
Gene Expression Regulation, Leukemic
Humans
Receptors, Antigen, B-Cell metabolism
Blotting, Western methods
Flow Cytometry methods
Leukemia, Lymphocytic, Chronic, B-Cell pathology
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1881
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 30350193
- Full Text :
- https://doi.org/10.1007/978-1-4939-8876-1_1