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Effect of Acyl Homoserine Lactone on Recombinant Production of Human Insulin-like Growth Factor-1 in Batch Culture of Escherichia coli.
- Source :
-
Protein and peptide letters [Protein Pept Lett] 2018; Vol. 25 (11), pp. 980-985. - Publication Year :
- 2018
-
Abstract
- Background: IGF-I as a human growth factor produced in Escherichia coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6 kDa. Up to now, E. coli expression system has been widely used as the host to produce rhIGF-1 with high yields. Acyl Homoserine Lactones (AHLs) are intercellular signaling molecules used in quorum sensing by Gram-negative bacteria. Quorum sensing is a cell density-dependent gene regulation process that allows bacterial cells to express specific genes only when signaling molecules reach the sufficient concentration.<br />Objective: For the first time, this study focuses on the N-hexanoyl-L- Homoserine Lactone (HHL) activity on increasing the cell growth and rh-IGF-1concentration in batch culture of E. coli.<br />Method: The maximum production of rhIGF-I was previously optimized in 32y culture medium at 32°C with 0.05 mM IPTG as inducer and 10 g/l glucose concentration. Under this condition, different amounts of HHL (0.001 µg/ml, 1 µg/ml, and 100µg/ml) were evaluated as an inducer for IGF-1 production.<br />Results: Generally, with increasing of HHL concentration, an increase in dry cell weight (2.45 mg/ml to 4.63 mg/ml) and IGF-I expression level (0.4 mg/ml to 0.77 mg/ml) was observed.<br />Conclusion: HHL or other types of AHLs can be considered as protein production inducer in bacterial expression systems through the quorum sensing pathways.<br /> (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
- Subjects :
- 4-Butyrolactone pharmacology
Cell Proliferation drug effects
Culture Techniques
Escherichia coli cytology
Humans
Insulin-Like Growth Factor I genetics
Quorum Sensing drug effects
Recombinant Proteins genetics
Transformation, Genetic drug effects
4-Butyrolactone analogs & derivatives
Escherichia coli drug effects
Escherichia coli genetics
Insulin-Like Growth Factor I biosynthesis
Recombinant Proteins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1875-5305
- Volume :
- 25
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Protein and peptide letters
- Publication Type :
- Academic Journal
- Accession number :
- 30338727
- Full Text :
- https://doi.org/10.2174/0929866525666181019150657