Back to Search
Start Over
Anaerobic respiration coupled with mitochondrial fatty acid synthesis in wax ester fermentation by Euglena gracilis.
- Source :
-
FEBS letters [FEBS Lett] 2018 Dec; Vol. 592 (24), pp. 4020-4027. Date of Electronic Publication: 2018 Oct 30. - Publication Year :
- 2018
-
Abstract
- In Euglena gracilis, wax ester fermentation produces ATP during anaerobiosis. Here, we report that anaerobic wax ester production is suppressed when the mitochondrial electron transport chain complex I is inhibited by rotenone, whereas it is increased by the uncoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP). The ADP/ATP ratio in anaerobic cells is elevated by treatment with either rotenone or CCCP. Gene silencing experiments indicate that acyl-CoA dehydrogenase, electron transfer flavoprotein (ETF), and rhodoquinone (RQ) participate in wax ester production. These results suggest that fatty acids are synthesized in mitochondria by the reversal of β-oxidation, where trans-2-enoyl-CoA is reduced mainly by acyl-CoA dehydrogenase using the electrons provided by NADH via the electron transport chain complex I, RQ, and ETF, and that ATP production is highly supported by anaerobic respiration utilizing trans-2-enoyl-CoA as a terminal electron acceptor.<br /> (© 2018 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
- Subjects :
- Acyl-CoA Dehydrogenase genetics
Acyl-CoA Dehydrogenase metabolism
Adenosine Diphosphate biosynthesis
Adenosine Triphosphate biosynthesis
Anaerobiosis
Esters chemistry
Euglena gracilis cytology
Euglena gracilis genetics
Mitochondria drug effects
RNA Interference
Rotenone pharmacology
Uncoupling Agents pharmacology
Waxes chemistry
Waxes metabolism
Cell Respiration
Esters metabolism
Euglena gracilis metabolism
Fatty Acids biosynthesis
Fermentation
Mitochondria metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 592
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Report
- Accession number :
- 30328102
- Full Text :
- https://doi.org/10.1002/1873-3468.13276