[Effect of the taurine on striatum tissue cell cycle and apoptosis in manganese exposed rats].

Objective: To elucidate the effect of taurine on neurotoxicity induced by Mn by investigating cell cycle and apoptosis in manganese exposed rats. Methods: 156 healthy male SD rats were randomly divided into 1 control group, 3 manganese exposure groups (10, 15, and 20 mg/kg respectively) , and 9 intervened groups based on orthogonal design, with 12 rats in each group. After 12 weeks of exposure, all rats were decapitated and striatums were removed, cell cycle was analyzed by flow cytometry, the apoptosis was detected by TUNEL, level of Mn was determined. Results: The striatum apoptosis index of the 3 dose groups exposed to Mn were significantly higher than control group ( P <0.05) . The striatum apoptosis index of the 9 intervened groups were significantly higher than control group ( P <0.05) . 150 and 200 mg/kg of taurine could decrease apoptosis index of the group exposed to 10、15、20 mg/kg of Mn ( P <0.05) . The striatum Mn content of the 3 dose groups exposed to Mn were significantly higher than that of the control group ( P <0.05) . The G0/G1 proportion of the 3 dose groups exposed to Mn were significantly lower than that of the control group ( P <0.05) , the S proportion of the 3 dose groups exposed to Mn were significantly higher than that of the control group ( P <0.05) . Conclusion: Mn could cause cell cycle arrest to S, increase level of apoptosis in striatum, to a certain extent, taurine can protect neurons from apoptosis induced by Mn.