High-frequency pulsed electron-electron double resonance spectroscopy on DNA duplexes using trityl tags and shaped microwave pulses.

Authors :: Kuzhelev A
Akhmetzyanov D
Denysenkov V
Shevelev G
Krumkacheva O
Bagryanskaya E
Prisner T
Source :: Physical chemistry chemical physics : PCCP [Phys Chem Chem Phys] 2018 Nov 07; Vol. 20 (41), pp. 26140-26144. Date of Electronic Publication: 2018 Oct 12.
Publication Year :: 2018
Abstract: Accurate distances between two trityl paramagnetic tags site-specifically attached to DNA duplexes were measured by pulsed electron-electron double resonance spectroscopy at 180 and 260 GHz microwave frequencies. Up to a threefold increase in the sensitivity of 260 GHz PELDOR measurements was achieved by using shaped broad-band microwave pulses.

Subjects :: Electron Spin Resonance Spectroscopy
Molecular Dynamics Simulation
Nucleic Acid Conformation
Temperature
DNA chemistry
Microwaves
Trityl Compounds chemistry

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