Back to Search
Start Over
Surveillance of transmitted HIV-1 antiretroviral drug resistance in the context of decentralized HIV care in Senegal and the Ebola outbreak in Guinea.
- Source :
-
BMC research notes [BMC Res Notes] 2018 Oct 12; Vol. 11 (1), pp. 723. Date of Electronic Publication: 2018 Oct 12. - Publication Year :
- 2018
-
Abstract
- Objectives: Disruption in HIV care provision may enhance the development and spread of drug resistance due to inadequate antiretroviral therapy. This study thus determined the prevalence of HIV-1 transmitted drug resistance (TDR) in settings of decentralized therapy and care in Senegal and, the Ebola outbreak in Guinea. Antiretroviral-naïve patients were enrolled following a modified WHO TDR Threshold Survey method, implemented in Senegal (January-March 2015) and Guinea (August-September 2015). Plasma and dried blood spots specimens, respectively from Senegalese (n = 69) and Guinean (n = 50) patients, were collected for direct sequencing of HIV-1 pol genes. The Stanford Calibrated Population Resistance program v6.0 was used for Surveillance Drug Resistance Mutations (SDRMs).<br />Results: Genotyping was successful from 54/69 (78.2%) and 31/50 (62.0%) isolates. In Senegal, TDR prevalence was 0% (mean duration since HIV diagnosis 4.08 ± 3.53 years). In Guinea, two patients exhibited SDRMs M184V (NRTI), T215F (TAM) and, G190A (NNRTI), respectively. TDR prevalence at this second site, however, could not be ascertained because of low sample size. Phylogenetic inference confirmed CRF02&#95;AG predominance in Senegal (62.96%) and Guinea (77.42%). TDR prevalence in Senegal remains extremely low suggesting improved control measures. Continuous surveillance in both settings is mandatory and, should be done closest to diagnosis/transmission time and with larger sample size.
- Subjects :
- Adult
Ebolavirus pathogenicity
Female
Genotyping Techniques
Guinea epidemiology
HIV Infections transmission
HIV-1 classification
HIV-1 isolation & purification
Humans
Male
Middle Aged
Mutation
Phylogeny
Public Health Surveillance methods
Senegal epidemiology
Anti-HIV Agents supply & distribution
Disease Outbreaks
Drug Resistance, Viral genetics
HIV Infections epidemiology
HIV-1 genetics
Hemorrhagic Fever, Ebola epidemiology
Subjects
Details
- Language :
- English
- ISSN :
- 1756-0500
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC research notes
- Publication Type :
- Academic Journal
- Accession number :
- 30309385
- Full Text :
- https://doi.org/10.1186/s13104-018-3804-9