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Derivative spectrophotometric studies on cytotoxic effects of carbon monoxide.
- Source :
-
Forensic science international [Forensic Sci Int] 1987 Feb; Vol. 33 (2), pp. 75-82. - Publication Year :
- 1987
-
Abstract
- Characteristics of cytochrome oxidase prepared from hearts of Sprague-Dawley male rats were studied with the use of the fourth derivative spectrophotometry in respect to the cytotoxic effects of carbon monoxide (CO). CO-exposed rats tended to show lower specific activities of cytochrome oxidase than control and recovered rats. Moreover, there was a significant difference in the fourth derivative spectral features of the enzyme: CO-exposed groups indicated peaks at 412 nm in the spectra while controls at 408 nm. This spectral difference seemed to reflect specific effect of CO on cytochrome oxidase, though such trace remained for not more than a day after death.
- Subjects :
- Animals
Carbon Monoxide blood
Carboxyhemoglobin biosynthesis
Electron Transport Complex IV metabolism
Hypoxia metabolism
Male
Oxygen Consumption drug effects
Rats
Rats, Inbred Strains
Spectrophotometry
Carbon Monoxide toxicity
Electron Transport Complex IV analysis
Hypoxia chemically induced
Subjects
Details
- Language :
- English
- ISSN :
- 0379-0738
- Volume :
- 33
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Forensic science international
- Publication Type :
- Academic Journal
- Accession number :
- 3030908
- Full Text :
- https://doi.org/10.1016/0379-0738(87)90142-3