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Escherichia coli mediated resistance of Entamoeba histolytica to oxidative stress is triggered by oxaloacetate.
- Source :
-
PLoS pathogens [PLoS Pathog] 2018 Oct 11; Vol. 14 (10), pp. e1007295. Date of Electronic Publication: 2018 Oct 11 (Print Publication: 2018). - Publication Year :
- 2018
-
Abstract
- Amebiasis, a global intestinal parasitic disease, is due to Entamoeba histolytica. This parasite, which feeds on bacteria in the large intestine of its human host, can trigger a strong inflammatory response upon invasion of the colonic mucosa. Whereas information about the mechanisms which are used by the parasite to cope with oxidative and nitrosative stresses during infection is available, knowledge about the contribution of bacteria to these mechanisms is lacking. In a recent study, we demonstrated that enteropathogenic Escherichia coli O55 protects E. histolytica against oxidative stress. Resin-assisted capture (RAC) of oxidized (OX) proteins coupled to mass spectrometry (OX-RAC) was used to investigate the oxidation status of cysteine residues in proteins present in E. histolytica trophozoites incubated with live or heat-killed E. coli O55 and then exposed to H2O2-mediated oxidative stress. We found that the redox proteome of E. histolytica exposed to heat-killed E. coli O55 is enriched with proteins involved in redox homeostasis, lipid metabolism, small molecule metabolism, carbohydrate derivative metabolism, and organonitrogen compound biosynthesis. In contrast, we found that proteins associated with redox homeostasis were the only OX-proteins that were enriched in E. histolytica trophozoites which were incubated with live E. coli O55. These data indicate that E. coli has a profound impact on the redox proteome of E. histolytica. Unexpectedly, some E. coli proteins were also co-identified with E. histolytica proteins by OX-RAC. We demonstrated that one of these proteins, E. coli malate dehydrogenase (EcMDH) and its product, oxaloacetate, are key elements of E. coli-mediated resistance of E. histolytica to oxidative stress and that oxaloacetate helps the parasite survive in the large intestine. We also provide evidence that the protective effect of oxaloacetate against oxidative stress extends to Caenorhabditis elegans.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Amebiasis drug therapy
Amebiasis metabolism
Amebiasis parasitology
Animals
Caenorhabditis elegans drug effects
Caenorhabditis elegans growth & development
Caenorhabditis elegans parasitology
Cells, Cultured
Entamoebiasis metabolism
Entamoebiasis parasitology
HeLa Cells
Humans
Intestine, Large drug effects
Intestine, Large metabolism
Intestine, Large parasitology
Macrophages cytology
Macrophages drug effects
Macrophages parasitology
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Entamoeba histolytica drug effects
Entamoebiasis drug therapy
Escherichia coli physiology
Oxaloacetic Acid pharmacology
Oxidative Stress drug effects
Protozoan Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7374
- Volume :
- 14
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- PLoS pathogens
- Publication Type :
- Academic Journal
- Accession number :
- 30308066
- Full Text :
- https://doi.org/10.1371/journal.ppat.1007295