Methods to Measure MDSC Immune Suppressive Activity In Vitro and In Vivo.

Authors :: Solito S
Pinton L
De Sanctis F
Ugel S
Bronte V
Mandruzzato S
Marigo I
Source :: Current protocols in immunology [Curr Protoc Immunol] 2019 Feb; Vol. 124 (1), pp. e61. Date of Electronic Publication: 2018 Oct 10.
Publication Year :: 2019
Abstract: This unit presents methods to assess the immunosuppressive properties of immunoregulatory cells of myeloid origin, such as myeloid-derived suppressor cells (MDSCs), both in vitro and in vivo in mice, as well as in biological samples from cancer patients. These methods could be adapted to test the impact of different suppressive populations on T cell activation, proliferation, and cytotoxic activity; moreover, they could be useful to assess the influence exerted by genetic modifications, chemical inhibitors, and drugs on immune suppressive pathways © 2018 by John Wiley & Sons, Inc.<br /> (© 2018 John Wiley & Sons, Inc.)