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Australian porcine clonal complex 10 (CC10) Escherichia coli belong to multiple sublineages of a highly diverse global CC10 phylogeny.
- Source :
-
Microbial genomics [Microb Genom] 2019 Mar; Vol. 5 (3). Date of Electronic Publication: 2018 Oct 10. - Publication Year :
- 2019
-
Abstract
- We recently identified clonal complex 10 (CC10) Escherichia coli as the predominant clonal group in two populations of healthy Australian food-production pigs. CC10 are highly successful, colonizing humans, food-production animals, fresh produce and environmental niches. Furthermore, E. coli within CC10 are frequently drug resistant and increasingly reported as human and animal extra-intestinal pathogens. In order to develop a high-resolution global phylogeny and determine the repertoire of antimicrobial-resistance genes, virulence-associated genes and plasmid types within this clonal group, we downloaded 228 publicly available CC10 short-read genome sequences for comparison with 20 porcine CC10 we have previously described. Core genome single nucleotide polymorphism phylogeny revealed a highly diverse global phylogeny consisting of multiple lineages that did not cluster by geography or source of the isolates. Australian porcine strains belonged to several of these divergent lineages, indicative that CC10 is present in these animals due to multiple colonization events. Differences in resistance gene and plasmid carriage between porcine strains and the global collection highlighted the role of lateral gene transfer in the evolution of CC10 strains. Virulence profiles typical of extra-intestinal pathogenic E. coli were present in both Australian porcine strains and the broader collection. As both the core phylogeny and accessory gene characteristics appeared unrelated to the geography or source of the isolates, it is likely that the global expansion of CC10 is not a recent event and may be associated with faecal carriage in humans.
- Subjects :
- Animals
Australia
Bacterial Typing Techniques
DNA, Bacterial genetics
Drug Resistance, Bacterial genetics
Escherichia coli isolation & purification
Escherichia coli Infections microbiology
Escherichia coli Infections veterinary
Escherichia coli Proteins genetics
Feces
Food Microbiology
Gene Transfer, Horizontal
Genome, Bacterial
Humans
Molecular Epidemiology
Plasmids
Swine Diseases microbiology
Virulence genetics
Whole Genome Sequencing
Escherichia coli classification
Escherichia coli genetics
Phylogeny
Swine microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 2057-5858
- Volume :
- 5
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Microbial genomics
- Publication Type :
- Academic Journal
- Accession number :
- 30303480
- Full Text :
- https://doi.org/10.1099/mgen.0.000225