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New antimicrobial compounds produced by endophytic Penicillium janthinellum isolated from Panax notoginseng as potential inhibitors of FtsZ.
- Source :
-
Fitoterapia [Fitoterapia] 2018 Nov; Vol. 131, pp. 35-43. Date of Electronic Publication: 2018 Oct 03. - Publication Year :
- 2018
-
Abstract
- A total of 180 fungal isolates, belonging to 20 genera and 47 species, were obtained from the roots, stems and leaves of Panax notoginseng. One isolate, the endophytic fungus Penicillium janthinellum SYPF 7899, displayed the strongest antibacterial activity and was studied for its production of secondary metabolites. In total, three new compounds, including rotational isomers 1a, 1b and 2 were isolated from the solid cultures of P. janthinellum, as well as eight known compounds (3-10). These structures were determined on the basis of 1D, 2D NMR and electronic circular dichroism (ECD) spectroscopic analyses as well as theoretical calculations. Compound 1 exhibited significant inhibitory activities against Bacillus subtilis and Staphylococcus aureus with MIC values of 15 and 18 μg/ml, respectively. The other compounds showed moderate or weak activities. In addition, morphological observation showed the rod-shaped cells of B. subtilis growing into long filaments, which reached 1.5- to 2-fold of the length of the original cells after treatment with compound 1. The coccoid cells of S. aureus exhibited a similar response and swelled to a 2-fold volume after treatment with compound 1. In silico molecular docking was explored to study the binding interactions between the compounds and the active sites of filamentous temperature-sensitive protein Z (FtsZ) from B. subtilis and S. aureus. Compound 1a, 1b and 2 showed high binding energies, strong H-bond interactions and hydrophobic interactions with FtsZ. Based on the antimicrobial activities, cellular phenotype observation and docking studies, compound 1 is considered to be a promising antimicrobial inhibitor of FtsZ.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Anti-Bacterial Agents pharmacology
Bacillus subtilis drug effects
China
Endophytes chemistry
Microbial Sensitivity Tests
Molecular Docking Simulation
Molecular Structure
Staphylococcus aureus drug effects
Anti-Bacterial Agents isolation & purification
Bacterial Proteins antagonists & inhibitors
Cytoskeletal Proteins antagonists & inhibitors
Panax notoginseng microbiology
Penicillium chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6971
- Volume :
- 131
- Database :
- MEDLINE
- Journal :
- Fitoterapia
- Publication Type :
- Academic Journal
- Accession number :
- 30291967
- Full Text :
- https://doi.org/10.1016/j.fitote.2018.10.006