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Identification of an aldehyde oxidase involved in indole-3-acetic acid synthesis in Bombyx mori silk gland.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2019 Jan; Vol. 83 (1), pp. 129-136. Date of Electronic Publication: 2018 Oct 05. - Publication Year :
- 2019
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Abstract
- Auxin is thought to be an important factor in the induction of galls by galling insects. We have previously shown that both galling and nongalling insects synthesize indole-3-acetic acid (IAA) from tryptophan (Trp) via two intermediates, indole-3-acetaldoxime (IAOx) and indole-3-acetaldehyde (IAAld). In this study, we isolated an enzyme that catalyzes the last step "IAAld → IAA" from a silk-gland extract of Bombyx mori. The enzyme, designated "BmIAO1", contains two 2Fe-2S iron-sulfur-cluster-binding domains, an FAD-binding domain, and a molybdopterin-binding domain, which are conserved in aldehyde oxidases. BmIAO1 causes the nonenzymatic conversion of Trp to IAAld and the enzymatic conversion of IAOx to IAA, suggesting that BmIAO1 alone is responsible for IAA production in B. mori. However, a detailed comparison of pure BmIAO1 and the crude silk-gland extract suggested the presence of other enzymes involved in IAA production from Trp. Abbreviations: BA: benzoic acid; CE: collision energy; CXP: collision cell exit potential; DP: declustering potential; IAA: indole-3-acetic acid; IBI1: IAA biosynthetic inhibitor-1; IAAld: indole-3-acetaldehyde; ICA: indole-3-carboxylic acid; IAOx: indole-3-acetaldoxime; IEtOH: indole-3-ethanol; LC-MS/MS: liquid chromatography-tandem mass spectrometry; Trp: tryptophan.
- Subjects :
- Aldehyde Oxidase chemistry
Aldehyde Oxidase isolation & purification
Amino Acid Sequence
Animals
Bombyx anatomy & histology
Bombyx enzymology
Catalysis
Chromatography methods
Electrophoresis, Polyacrylamide Gel
Sequence Homology, Amino Acid
Spectrophotometry, Ultraviolet methods
Substrate Specificity
Tandem Mass Spectrometry methods
Aldehyde Oxidase metabolism
Bombyx physiology
Indoleacetic Acids metabolism
Plant Growth Regulators metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 83
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 30286706
- Full Text :
- https://doi.org/10.1080/09168451.2018.1525275