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The influence of porcine epidemic diarrhea virus on pig small intestine mucosal epithelial cell function.
- Source :
-
Archives of virology [Arch Virol] 2019 Jan; Vol. 164 (1), pp. 83-90. Date of Electronic Publication: 2018 Oct 04. - Publication Year :
- 2019
-
Abstract
- Porcine epidemic diarrhea (PED) is a highly contagious, acute enteric tract infectious disease of pigs (Sus domesticus) caused by porcine epidemic diarrhea virus (PEDV). PED is characterized by watery diarrhea, dehydration, weight loss, vomiting and death. PEDV damages pig intestinal epithelial tissue, causing intestinal hyperemia and atrophy of intestinal villi, with formation of intestinal epithelial cell cytoplasmic vacuoles. Since pig small intestinal epithelial cells (IECs) are target cells of PEDV infection, IEC cells were utilized as a model for studying changes in cellular activities post-PEDV infection. Monitoring of Na <superscript>+</superscript> -K <superscript>+</superscript> -ATPase and Ca <superscript>2+</superscript> -Mg <superscript>2+</superscript> -ATPase activities demonstrated that PEDV infection decreased these activities. In addition, IECs proliferation was shown to decrease after PEDV infection using an MTT assay. Moreover, IECs apoptosis detected by flow cytometry with propidium iodide (PI) staining was clearly shown to increase relative to the control group. Meanwhile, animal experiments indicated that PEDV virulence for IEC cells was greater than viral virulence for Vero cells, although this may be due to viral attenuation after numerous passages in the latter cell line. Collectively, these studies revealed viral pathogenic mechanisms in PEDV-infected IECs and offer a theoretical basis for PEDV prevention and control.
- Subjects :
- Animals
Ca(2+) Mg(2+)-ATPase metabolism
Cell Survival
Chlorocebus aethiops
Coronavirus Infections pathology
Coronavirus Infections virology
Epithelial Cells pathology
Intestine, Small virology
Sodium-Potassium-Exchanging ATPase metabolism
Swine
Vero Cells
Virulence
Coronavirus Infections veterinary
Epithelial Cells virology
Intestinal Mucosa cytology
Intestine, Small pathology
Porcine epidemic diarrhea virus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1432-8798
- Volume :
- 164
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 30284628
- Full Text :
- https://doi.org/10.1007/s00705-018-4061-x