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Cold shock Y-box binding protein-1 acetylation status in monocytes is associated with systemic inflammation and vascular damage.
- Source :
-
Atherosclerosis [Atherosclerosis] 2018 Nov; Vol. 278, pp. 156-165. Date of Electronic Publication: 2018 Sep 22. - Publication Year :
- 2018
-
Abstract
- Background and Aims: In dialysis patients, vascular morbidities are highly prevalent and linked to leukocyte extravasation, especially of polarized monocytes. Experimental data demonstrate that phenotypic changes in monocytes require Y-box binding protein-1 (YB-1) upregulation.<br />Methods: We determined YB-1 expression in circulating and vessel-invading monocytes from healthy controls and dialysis patients to correlate results with intima plaque formation and systemic inflammation.<br />Results: Compared to healthy subjects, dialysis patients have fewer classical and more intermediate and non-classical monocytes. Post-translationally modified YB-1 (lysine 301/304 acetylation) is detected at high levels in the nucleus of adherent and invading CD14 <superscript>+</superscript> CD68 <superscript>+</superscript> monocytes from umbilical cord and atherosclerosis-prone vessels. The content of non-acetylated YB-1 is significantly decreased (p < 0.001), whereas acetylated YB-1 is correspondingly increased (p < 0.001) throughout all monocyte subpopulations, such that the overall content remains unchanged.<br />Conclusions: In dialysis patients the YB-1 acetylation status is higher with prevailing diabetes and intima plaque formation. Pro-inflammatory mediators TNFα, IL-6, uPAR, CCL2, M-CSF, progranulin, ANP, and midkine, as well as anti-inflammatory IL-10 are significantly increased in dialysis patients, emphasizing a systemic inflammatory milieu. Strong positive correlations of monocytic YB-1 content are seen with ANP, IP-10, IL-6, and IL-10 serum levels. This is the first study demonstrating an association of cold shock protein YB-1 expression with inflammation in hemodialysis patients.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Acetylation
Adult
Aged
Aged, 80 and over
Antigens, CD metabolism
Antigens, Differentiation, Myelomonocytic metabolism
Atherosclerosis
Carotid Intima-Media Thickness
Cold-Shock Response
Comorbidity
Cytokines blood
Epitopes
Female
Humans
Ligands
Lipopolysaccharide Receptors metabolism
Male
Middle Aged
Protein Processing, Post-Translational
Renal Dialysis adverse effects
Tunica Intima pathology
Umbilical Cord metabolism
Young Adult
Inflammation blood
Monocytes metabolism
Vascular Diseases blood
Y-Box-Binding Protein 1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1484
- Volume :
- 278
- Database :
- MEDLINE
- Journal :
- Atherosclerosis
- Publication Type :
- Academic Journal
- Accession number :
- 30278358
- Full Text :
- https://doi.org/10.1016/j.atherosclerosis.2018.09.020