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Beneficial effects of Saccharomyces cerevisiae RC016 in weaned piglets: in vivo and ex vivo analysis.
- Source :
-
Beneficial microbes [Benef Microbes] 2019 Feb 08; Vol. 10 (1), pp. 33-42. Date of Electronic Publication: 2018 Oct 02. - Publication Year :
- 2019
-
Abstract
- Probiotics represents an alternative to replace antibiotics as growth promoters in animal feed and are able to control enteric bacterial diseases and to improve gut immunity. Saccharomyces cerevisiae RC016 showed previously inhibition/coagregation of pathogens) and mycotoxins adsorbent ability (aflatoxin B <subscript>1</subscript> , ochratoxin A and zearalenone). The aim of this work was to evaluate beneficial properties of S. cerevisiae RC016 in a non-inflammatory in vivo model in weaned piglets and in an intestinal inflammation ex vivo model induced by the mycotoxin deoxynivalenol (DON). Secretory immunoglobulin A (s-IgA) levels, intestinal cytokines, goblet cells and production parameters were evaluated in a pig model. For the in vivo assays, twelve pigs were weaned at 21 days and assigned to two groups: Control (n=6) and Yeast (n=6). Animals received yeast strain for three weeks. After 22 days the small intestine was recovered for determination of goblet cells and s-IgA. For the ex vivo assay, jejunal explants were obtained from 5 weeks old crossbred piglets and treated as follow: (1) control; (2) treated for 3 h with 10 μM DON used as an inflammatory stressor; (3) incubated with 10 <superscript>7</superscript> cfu/ml yeast strain; (4) pre-incubated 1 h with 10 <superscript>7</superscript> cfu/ml yeast strain and then treated for 3 h with 10 μM DON. CCL20, interleukin (IL)-1β, IL-8 and IL-22 gene expression was determined by qPCR. Oral administration of S. cerevisiae RC016 increased s-IgA, the number of goblet cells in small intestine and all the growth parameters measured. In the ex vivo model, the cytokine profile studied showed a potential anti-inflammatory effect of the administration of the yeast. In conclusion, S. cerevisiae RC016 is a promising candidate for feed additives formulation to improve animal growth and gut immune system. This yeast strain could be able to improve the gut health through counteracting the weaning-associated intestinal inflammation in piglets.
- Subjects :
- Animal Feed analysis
Animals
Cecum microbiology
Cytokines genetics
Enteritis chemically induced
Gene Expression
Goblet Cells cytology
Immunoglobulin A metabolism
Intestines immunology
Male
Models, Biological
Swine
Trichothecenes poisoning
Weaning
Enteritis prevention & control
Enteritis therapy
Food Additives administration & dosage
Probiotics administration & dosage
Saccharomyces cerevisiae physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1876-2891
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Beneficial microbes
- Publication Type :
- Academic Journal
- Accession number :
- 30274522
- Full Text :
- https://doi.org/10.3920/BM2018.0023