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Mechanism of HN‑3 cell apoptosis induced by carboplatin: Combination of mitochondrial pathway associated with Ca2+ and the nucleus pathways.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2018 Dec; Vol. 18 (6), pp. 4978-4986. Date of Electronic Publication: 2018 Sep 24. - Publication Year :
- 2018
-
Abstract
- Laryngeal carcinomas have been recognized as a serious health threat worldwide. In the present study, the mechanism of apoptosis in HN‑3 cells induced by carboplatin (CBCDA), a widely used anti‑cancer drug, was investigated. The pro‑apoptotic effect of CBCDA in HN‑3 cells was demonstrated to be time‑ and dose‑dependent. Therefore, the stages of apoptosis were investigated in chronological order. The results demonstrated that excessive generation of cytosolic Ca2+ in HN‑3 cells was initially triggered when cells were exposed to CBCDA, followed by the appearance of mitochondrial depolarization and oxidative stress, leading to the release of apoptosis‑inducing factor. At later stages, expression of caspase‑8 was increased due to the apoptotic signals originating from CBCDA‑induced DNA damage, as well as caspase‑9 and poly ADP ribose polymerase (PARP) expression upregulation. Glutathione decreased the available CBDCA concentration, decreased apoptosis and alleviating oxidative stress, thus reducing the actual effective concentration. Mechanistic research may benefit the rational design of more efficient therapeutic strategies as well as development of novel platinum‑based agents.
- Subjects :
- Calcium metabolism
Caspases metabolism
Cell Line, Tumor
Cell Nucleus metabolism
Cell Survival
Humans
Membrane Potential, Mitochondrial drug effects
Mitochondria drug effects
Mitochondria metabolism
Models, Biological
Signal Transduction drug effects
Antineoplastic Agents pharmacology
Apoptosis drug effects
Carboplatin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 18
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 30272304
- Full Text :
- https://doi.org/10.3892/mmr.2018.9507