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Homosalate aggravates the invasion of human trophoblast cells as well as regulates intracellular signaling pathways including PI3K/AKT and MAPK pathways.
- Source :
-
Environmental pollution (Barking, Essex : 1987) [Environ Pollut] 2018 Dec; Vol. 243 (Pt B), pp. 1263-1273. Date of Electronic Publication: 2018 Sep 21. - Publication Year :
- 2018
-
Abstract
- Homosalate is an organic ultraviolet filter used in most sunscreens but has been reported to be toxic to marine organisms. The estrogenic activity of homosalate has also been reported, but its endocrine-disrupting effect remains unclear. Although homosalate has been detected in human placental tissues, its effect on the survival of human trophoblast cells needs to be investigated. Therefore, in this study, we evaluated if HTR8/SVneo, a human trophoblast cell line, treated with homosalate showed decreasing proliferative activity in a dose-dependent manner. Homosalate promoted the death of HTR8/SVneo cells with elevated lipid peroxidation and intracellular Ca <superscript>2+</superscript> concentration. It also induced endoplasmic reticulum stress and mitochondrial morphological disturbances associated with the differentiation of human trophoblast cells. However, when the intracellular Ca <superscript>2+</superscript> or reactive oxygen species were removed using BAPTA-AM or N-acetyl-L-cysteine (NAC), the cell proliferation suppressed by homosalate was restored. Homosalate also significantly inhibited the invasion of HTR8/SVneo cells. Furthermore, it modulated phosphoinositide 3-kinase (PI3K)/AKT and mitogen-activated protein kinase (MAPK) signaling pathways, which were involved in the cross-talk between both signaling pathways in HTR8/SVneo cells. Thus, homosalate adversely affects the survival, proliferation, and invasiveness of human trophoblast cells and therefore pregnant women should practice caution while using personal care products containing homosalate.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Subjects :
- Cell Line
Cell Movement
Cell Proliferation drug effects
Endoplasmic Reticulum Stress
Humans
Mitochondria drug effects
Mitogen-Activated Protein Kinases
Phosphatidylinositol 3-Kinase metabolism
Phosphatidylinositol 3-Kinases metabolism
Proto-Oncogene Proteins c-akt metabolism
Reactive Oxygen Species metabolism
Signal Transduction drug effects
Trophoblasts
Salicylates toxicity
Sunscreening Agents toxicity
Toxicity Tests
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6424
- Volume :
- 243
- Issue :
- Pt B
- Database :
- MEDLINE
- Journal :
- Environmental pollution (Barking, Essex : 1987)
- Publication Type :
- Academic Journal
- Accession number :
- 30267922
- Full Text :
- https://doi.org/10.1016/j.envpol.2018.09.092