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TREM1: A positive regulator for inflammatory response via NF-κB pathway in A549 cells infected with Mycoplasma pneumoniae.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2018 Nov; Vol. 107, pp. 1466-1472. Date of Electronic Publication: 2018 Sep 04. - Publication Year :
- 2018
-
Abstract
- Herein, we found that serum content of the triggering receptor expressed on myeloid cells-1 (TREM1) was increased, and positively correlated with Mycoplasma pneumoniae (MP)-DNA in children with MP infection. In this study, A549 cells, known as human lung epithelial cells, were co-cultured with 10 <superscript>7</superscript> CCU/ml of MP to established in vitro model of MP infection. We studied the roles of TREM1 in inflammatory response of A549 cell by regulating the secretions of cytokine interleukin (IL)-8 and tumor necrosis factor (TNF)-α in cell culture supernatants. Moreover, transcriptional activity of nuclear factor kappa B (NF-кB) was assessed by measuring protein levels of NF-кB in the cytoplasm and nuclear. Our data suggested that sanguinarine chloride significantly decreased TREM1 expression, and alleviated inflammatory response of MP-infected A549 cells via preventing NF-кB nuclear translocation. To study the roles of TREM1 in inflammatory regulation in MP-infected A549 cells and the underlying mechanisms, we established TREM1 overexpression transfected A549 cells. PDTC was used for inhibiting NF-кB nuclear translocation. We found that TREM1 overexpression induced server inflammatory response of A549 cells. Besides, TREM1 overexpression attenuated anti-inflammatory effects of sanguinarine chloride in MP-infected cells. More importantly, pro-inflammatory effects of TREM1 overexpression was significantly reversed with additional PDTC treatment in MP-infected cells treated with sanguinarine chloride, suggesting that TREM1 was a pro-inflammatory factor via regulating NF-кB nuclear translocation in MP-infected A549 cells.<br /> (Copyright © 2018 Elsevier Masson SAS. All rights reserved.)
- Subjects :
- A549 Cells
Active Transport, Cell Nucleus
Benzophenanthridines pharmacology
Epithelial Cells pathology
Gene Expression Regulation
Humans
Inflammation genetics
Inflammation microbiology
Inflammation pathology
Interleukin-8 metabolism
Isoquinolines pharmacology
Lung cytology
Lung microbiology
Lung pathology
Mycoplasma pneumoniae isolation & purification
Pneumonia, Mycoplasma genetics
Tumor Necrosis Factor-alpha metabolism
NF-kappa B metabolism
Pneumonia, Mycoplasma pathology
Triggering Receptor Expressed on Myeloid Cells-1 blood
Triggering Receptor Expressed on Myeloid Cells-1 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 107
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 30257363
- Full Text :
- https://doi.org/10.1016/j.biopha.2018.07.176