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Generation and characterization of thiol-deficient Mycobacterium tuberculosis mutants.
- Source :
-
Scientific data [Sci Data] 2018 Sep 25; Vol. 5, pp. 180184. Date of Electronic Publication: 2018 Sep 25. - Publication Year :
- 2018
-
Abstract
- Mycothiol (MSH) and ergothioneine (ERG) are thiols able to compensate for each other to protect mycobacteria against oxidative stress. Gamma-glutamylcysteine (GGC), another thiol and an intermediate in ERG biosynthesis has detoxification abilities. Five enzymes are involved in ERG biosynthesis, namely EgtA, EgtB, EgtC, EgtD and EgtE. The role of these enzymes in the production of ERG had been unclear. On the other hand, the enzyme MshA is known to be essential for MSH biosynthesis. In this manuscript, we describe the raw data of the generation and characterization of Mycobacterium tuberculosis (M.tb) mutants harbouring a deletion of the gene coding for each of these enzymes, and the raw data of the phenotypic characterization of the obtained thiol-deficient M.tb mutants. High throughput screening (HTS) of off-patent drugs and natural compounds revealed few compounds that displayed a higher activity against the thiol-deficient mutants relative to the wild-type strain. The mode of action of these drugs was further investigated. Raw data displaying these results are described here.
- Subjects :
- Bacterial Proteins chemistry
Bacterial Proteins genetics
Mutation
Oxidative Stress genetics
Sulfhydryl Compounds
Cysteine deficiency
Cysteine genetics
Dipeptides deficiency
Dipeptides genetics
Ergothioneine deficiency
Ergothioneine genetics
Glycopeptides deficiency
Glycopeptides genetics
Inositol deficiency
Inositol genetics
Mycobacterium tuberculosis chemistry
Mycobacterium tuberculosis genetics
Mycobacterium tuberculosis isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 2052-4463
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- Scientific data
- Publication Type :
- Academic Journal
- Accession number :
- 30251996
- Full Text :
- https://doi.org/10.1038/sdata.2018.184