Back to Search
Start Over
Frozen blood clots can be used for the diagnosis of distinct Plasmodium species in man and non-human primates from the Brazilian Atlantic Forest.
- Source :
-
Malaria journal [Malar J] 2018 Sep 24; Vol. 17 (1), pp. 338. Date of Electronic Publication: 2018 Sep 24. - Publication Year :
- 2018
-
Abstract
- Background: Zoonotic infections with epidemic potential, as non-human primate malaria and yellow fever (YF), can overlap geographically. Optimizing a small blood sample for diagnosis and surveillance is of great importance. Blood are routinely collected for YF diagnosis and blood clots usually discarded after serum obtention. Aiming to take sample advantage, the sensitivity of a PCR using extracted DNA from long-term frozen clots from human and non-human primates for detection of Plasmodium spp. in low parasitaemia conditions was assayed.<br />Results: Malaria diagnosis with DNA extracted from blood clots generated results in agreement with samples obtained with whole blood, including mixed Plasmodium vivax/simium and Plasmodium malariae/brasilianum infections.<br />Conclusion: Blood clots from human and non-human primates may be an important and low cost source of DNA for malaria surveillance in the Atlantic Forest.
- Subjects :
- Animals
Brazil
Coinfection diagnosis
Coinfection parasitology
Humans
Malaria diagnosis
Malaria parasitology
Malaria, Vivax diagnosis
Malaria, Vivax parasitology
Malaria, Vivax veterinary
Monkey Diseases parasitology
Plasmodium classification
Plasmodium malariae isolation & purification
Plasmodium vivax isolation & purification
Thrombosis parasitology
Alouatta
Callithrix
Coinfection veterinary
Malaria veterinary
Monkey Diseases diagnosis
Plasmodium isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1475-2875
- Volume :
- 17
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Malaria journal
- Publication Type :
- Academic Journal
- Accession number :
- 30249260
- Full Text :
- https://doi.org/10.1186/s12936-018-2485-0