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CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1850, pp. 123-131. - Publication Year :
- 2018
-
Abstract
- Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the two most popular mammalian hosts for the production of recombinant proteins. In this chapter the suspension cultivation and transfection of these cells in small-scale disposable bioreactors is described. The TubeSpin bioreactor 50 and TubeSpin bioreactor 600 are designed for the cultivation of suspension cells by orbital shaking and have maximum working volumes of about 15 mL and 400 mL, respectively.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1850
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 30242684
- Full Text :
- https://doi.org/10.1007/978-1-4939-8730-6_9