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Phosphoethanolamine cellulose enhances curli-mediated adhesion of uropathogenic Escherichia coli to bladder epithelial cells.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2018 Oct 02; Vol. 115 (40), pp. 10106-10111. Date of Electronic Publication: 2018 Sep 19. - Publication Year :
- 2018
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Abstract
- Uropathogenic Escherichia coli (UPEC) are the major causative agents of urinary tract infections, employing numerous molecular strategies to contribute to adhesion, colonization, and persistence in the bladder niche. Identifying strategies to prevent adhesion and colonization is a promising approach to inhibit bacterial pathogenesis and to help preserve the efficacy of available antibiotics. This approach requires an improved understanding of the molecular determinants of adhesion to the bladder urothelium. We designed experiments using a custom-built live cell monolayer rheometer (LCMR) to quantitatively measure individual and combined contributions of bacterial cell surface structures [type 1 pili, curli, and phosphoethanolamine (pEtN) cellulose] to bladder cell adhesion. Using the UPEC strain UTI89, isogenic mutants, and controlled conditions for the differential production of cell surface structures, we discovered that curli can promote stronger adhesive interactions with bladder cells than type 1 pili. Moreover, the coproduction of curli and pEtN cellulose enhanced adhesion. The LCMR enables the evaluation of adhesion under high-shear conditions to reveal this role for pEtN cellulose which escaped detection using conventional tissue culture adhesion assays. Together with complementary biochemical experiments, the results support a model wherein cellulose serves a mortar-like function to promote curli association with and around the bacterial cell surface, resulting in increased bacterial adhesion strength at the bladder cell surface.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Bacterial Proteins genetics
Cell Line
Cellulose pharmacology
Epithelial Cells microbiology
Epithelial Cells ultrastructure
Ethanolamines pharmacology
Humans
Urinary Bladder microbiology
Urinary Bladder ultrastructure
Uropathogenic Escherichia coli pathogenicity
Uropathogenic Escherichia coli ultrastructure
Urothelium microbiology
Urothelium ultrastructure
Bacterial Adhesion drug effects
Bacterial Proteins metabolism
Cellulose adverse effects
Epithelial Cells metabolism
Ethanolamines adverse effects
Urinary Bladder metabolism
Uropathogenic Escherichia coli metabolism
Urothelium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 115
- Issue :
- 40
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 30232265
- Full Text :
- https://doi.org/10.1073/pnas.1801564115