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Inhibition of epithelial‑mesenchymal transition in gastric cancer cells by miR‑711‑mediated downregulation of CD44 expression.
- Source :
-
Oncology reports [Oncol Rep] 2018 Nov; Vol. 40 (5), pp. 2844-2853. Date of Electronic Publication: 2018 Sep 04. - Publication Year :
- 2018
-
Abstract
- Gastric cancer is a common malignancy worldwide. The prognosis of early stage gastric cancer patients has significantly improved in recent years. However, in progressive stage gastric cancer patients, the prognosis remains relatively poor due to tumor metastases. In our previous study, we showed that the expression of miR‑711 in gastric cancer tissues is low, and restoration of miR‑711 inhibited the invasion and migration and the occurrence of epithelial‑mesenchymal transition (EMT) in gastric cancer cells. Yet, the mechanisms involved in these processes remain unknown. In the present study, we demonstrated that miR‑711‑mediated downregulation of CD44 expression inhibited EMT of gastric cancer cells in vitro and in vivo by downregulating vimentin protein expression and upregulating E‑cadherin protein expression through transfection, qRT‑PCR and western blotting. Therefore, miR‑711 may provide a promising target for EMT‑related therapy for gastric cancer.
- Subjects :
- Animals
Cell Line, Tumor
Cell Movement genetics
Cell Proliferation genetics
Epithelial-Mesenchymal Transition genetics
Gene Expression Regulation, Neoplastic
Humans
Mice
Neoplasm Invasiveness genetics
Neoplasm Staging
Prognosis
Stomach Neoplasms pathology
Stomach Neoplasms therapy
Transfection
Xenograft Model Antitumor Assays
Cadherins genetics
Hyaluronan Receptors genetics
MicroRNAs genetics
Stomach Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2431
- Volume :
- 40
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 30226620
- Full Text :
- https://doi.org/10.3892/or.2018.6681