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Satellite cells delivered in their niche efficiently generate functional myotubes in three-dimensional cell culture.
- Source :
-
PloS one [PLoS One] 2018 Sep 17; Vol. 13 (9), pp. e0202574. Date of Electronic Publication: 2018 Sep 17 (Print Publication: 2018). - Publication Year :
- 2018
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Abstract
- Biophysical/biochemical cues from the environment contribute to regulation of the regenerative capacity of resident skeletal muscle stem cells called satellites cells. This can be observed in vitro, where muscle cell behaviour is influenced by the particular culture substrates and whether culture is performed in a 2D or 3D environment, with changes including morphology, nuclear shape and cytoskeletal organization. To create a 3D skeletal muscle model we compared collagen I, Fibrin or PEG-Fibrinogen with different sources of murine and human myogenic cells. To generate tension in the 3D scaffold, biomaterials were polymerised between two flexible silicone posts to mimic tendons. This 3D culture system has multiple advantages including being simple, fast to set up and inexpensive, so providing an accessible tool to investigate myogenesis in a 3D environment. Immortalised human and murine myoblast lines, and primary murine satellite cells showed varying degrees of myogenic differentiation when cultured in these biomaterials, with C2 myoblasts in particular forming large multinucleated myotubes in collagen I or Fibrin. However, murine satellite cells retained in their niche on a muscle fibre and embedded in 3D collagen I or Fibrin gels generated aligned, multinucleated and contractile myotubes.<br />Competing Interests: IM and TE are co-founders of EHT Technologies GmbH, Hamburg, a UKE spin-off commercializing the materials for the making and analysis of engineered heart tissue. This does not alter our adherence to PLOS ONE policies on sharing data and materials. JP, PSZ and NF declare no competing interests.
- Subjects :
- Animals
Cell Differentiation
Cells, Cultured
Collagen Type I chemistry
Cytoskeleton metabolism
Fibrin chemistry
Fibrinogen chemistry
Humans
Mice
Muscle Development
Tissue Engineering methods
Cell Culture Techniques methods
Muscle Fibers, Skeletal cytology
Satellite Cells, Skeletal Muscle cytology
Tissue Scaffolds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 13
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 30222770
- Full Text :
- https://doi.org/10.1371/journal.pone.0202574