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Isolation and identification of Treponema pedis and Treponema phagedenis-like organisms from bovine digital dermatitis lesions found in dairy cattle in Turkey.
- Source :
-
Journal of dairy science [J Dairy Sci] 2018 Nov; Vol. 101 (11), pp. 10317-10326. Date of Electronic Publication: 2018 Sep 13. - Publication Year :
- 2018
-
Abstract
- The isolation and identification of microorganisms associated with digital dermatitis (DD) in Turkish dairy cattle was investigated using punch skin biopsy samples from typical clinical lesions; they were collected from dairy farms and abattoirs in 5 different geographical locations in Turkey. Morphological characteristics and flagellation types were examined using a transmission electron microscope, and their enzyme profiles by enzyme activation kits; their catalase reaction characteristics were evaluated by the addition of 3% H <subscript>2</subscript> O <subscript>2</subscript> . Their phylogenies were identified using 16S rRNA and the results compared with known gene bank data. Bacterial cells were 5.0 to 18.2 µm long, 0.2 to 0.5 µm wide, and their minimum number of periplasmic flagellum was 4 (4:8:4) with a maximum of 8 (8:16:8). All isolates were catalase negative. Of the spirochetes isolated and identified, group I organisms showed close similarity with Treponema pedis (99% genetic homology), whereas those in group II were similar to Treponema phagedenis (98% homology). This is the first report of specific sub-groups of Treponema spp., isolated from Turkish dairy cattle presenting with DD lesions, being associated with this disease; these morphotypes were similar to those found globally in housed dairy cattle units and are probably significant microorganisms associated with the aetiopathogensis of this infectious disease causing acute bovine lameness. These results suggest that the distribution of DD-associated treponemes is not specific to particular geographic regions of Turkey.<br /> (Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cattle
Cattle Diseases epidemiology
Dairying
Digital Dermatitis epidemiology
Female
Phylogeny
RNA, Ribosomal, 16S genetics
Treponema genetics
Treponemal Infections epidemiology
Treponemal Infections microbiology
Turkey epidemiology
Cattle Diseases microbiology
Digital Dermatitis microbiology
Treponema isolation & purification
Treponemal Infections veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1525-3198
- Volume :
- 101
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of dairy science
- Publication Type :
- Academic Journal
- Accession number :
- 30219415
- Full Text :
- https://doi.org/10.3168/jds.2017-14227