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Induction of cells with prostate cancer stem-like properties from mouse induced pluripotent stem cells via conditioned medium.

Authors :
Xu N
Li X
Watanabe M
Ueki H
Hu H
Li N
Araki M
Wada K
Xu A
Liu C
Nasu Y
Huang P
Source :
American journal of cancer research [Am J Cancer Res] 2018 Aug 01; Vol. 8 (8), pp. 1624-1632. Date of Electronic Publication: 2018 Aug 01 (Print Publication: 2018).
Publication Year :
2018

Abstract

Cancer stem cells (CSCs) that closely correlated with tumor growth, metastasis, provide a plausible explanation for chemoresistance and cancer relapse. CSCs are usually isolated and enriched from carcinoma cells, which is inconvenient, low-efficient, and even unreliable. Here, we converted mouse induced pluripotent stem cells (miPSCs) into prostate cancer stem-like cells with carcinoma microenvironment following exposure to conditioned medium (CM) derived from RM9, a mouse prostate cancer cell line. These transformed cells, termed as miPS-RM9CM, displayed CSCs properties, including spheroids morphology and expression of both stemness genes and cancer stem cells surface markers, such as Oct3/4, Sox2, Nanog, Klf-4, c-Myc, CD44, and CD133. In addition, in vivo transplantation experiment was performed to confirm the tumorigenicity. Furthermore, we used the model to assess conventional chemotherapeutic agent, docetaxel. The results showed that miPS-RM9CM cells exhibited increased resistance to docetaxel, however, high susceptibility to the cancer cell stemness inhibitor I (BBI-608). Our current study demonstrates that CM from cultured RM9 cells play a crucial role in the determination of cell fate from miPSCs to cancer stem-like cells and provide a potentially valuable system for the study of CSCs.<br />Competing Interests: None.

Details

Language :
English
ISSN :
2156-6976
Volume :
8
Issue :
8
Database :
MEDLINE
Journal :
American journal of cancer research
Publication Type :
Academic Journal
Accession number :
30210930