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Cyberlindnera fabianii fungaemia outbreak in preterm neonates in Kuwait and literature review.
- Source :
-
Mycoses [Mycoses] 2019 Jan; Vol. 62 (1), pp. 51-61. Date of Electronic Publication: 2018 Oct 15. - Publication Year :
- 2019
-
Abstract
- Background: Cyberlindnera fabianii has rarely been reported as a human pathogen. Here, we describe an outbreak of C. fabianii fungaemia involving 10 preterm neonates during a seven-month period in Kuwait and review the published reports.<br />Methods: Blood cultures were processed, and yeast isolates were initially identified by ID 32 C and/or VITEK 2. Molecular identification was done by PCR sequencing of internally transcribed spacer (ITS) region and D1/D2 domains of rDNA. Fingerprinting was performed with microsatellite-based and minisatellite-based primers to examine genetic relatedness among the isolates. Antifungal susceptibility testing of the isolates was done by Etest.<br />Findings: All infected neonates were preterm, received prior antibiotics and had an intravascular catheter in place. All bloodstream isolates were initially identified as Candida utilis by ID 32 C and/or VITEK 2 and showed reduced susceptibility to triazoles. PCR sequencing of rDNA identified all isolates as Cyberlindnera fabianii. Fingerprinting studies yielded identical patterns indicating clonality. One neonate died before treatment, one died during treatment, and eight neonates survived treatment with amphotericin B with/without fluconazole or caspofungin. Source of infection remained unknown despite surveillance cultures.<br />Conclusion: The outbreak highlights emergence of C. fabianii as a neonatal pathogen and reinforces importance of molecular methods in its accurate identification.<br /> (© 2018 Blackwell Verlag GmbH.)
- Subjects :
- Antifungal Agents pharmacology
Blood microbiology
Catheter-Related Infections epidemiology
Catheter-Related Infections microbiology
Cluster Analysis
Cross Infection epidemiology
Cross Infection microbiology
DNA, Fungal chemistry
DNA, Fungal genetics
DNA, Intergenic chemistry
DNA, Intergenic genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Disk Diffusion Antimicrobial Tests
Female
Humans
Infant, Newborn
Kuwait epidemiology
Male
Minisatellite Repeats
Molecular Epidemiology
Molecular Typing
Mycological Typing Techniques
Phylogeny
RNA, Ribosomal genetics
Saccharomycetales drug effects
Saccharomycetales genetics
Sequence Analysis, DNA
Disease Outbreaks
Fungemia epidemiology
Fungemia microbiology
Infant, Premature
Saccharomycetales classification
Saccharomycetales isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1439-0507
- Volume :
- 62
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Mycoses
- Publication Type :
- Academic Journal
- Accession number :
- 30184277
- Full Text :
- https://doi.org/10.1111/myc.12846