Back to Search
Start Over
Characterization of arabinogalactans from Larix principis-rupprechtii and their effects on NO production by macrophages.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2018 Nov 15; Vol. 200, pp. 408-415. Date of Electronic Publication: 2018 Aug 09. - Publication Year :
- 2018
-
Abstract
- Arabinogalactans are a source of dietary fiber with health benefits. In this work, two arabinogalactans assigned as AGW and AGS were isolated from Larix principis-rupprechtii, and characterized by gel permeation chromatography (GPC), monosaccharide analysis, methylation analysis and NMR spectroscopy analysis. The average molecular weights of AGW and AGS were 1.53 × 10 <superscript>4</superscript> and 1.84 × 10 <superscript>4</superscript> Da, respectively. Methylation analysis and NMR spectra suggested that AGW and AGS have a 1,3-linked Galp backbone, branched at C-6 with 1,6-linked Galp side residues. The Ara residues were substituted at C-6 of 1,6-linked Galp consisting of α-L-Araf-(1→3)-α-L-Araf-(1→6)-β-D-Galp-(1→ and β-L-Arap-(1→6)-β-D-Galp-(1→. Significantly, AGS (0.74%) was shown to contain 25 times more uronic acid than AGW (0.03%), which demonstrated a polyelectrolyte effect. Application of these two polysaccharides to macrophage RAW 264.7 cells was shown to increase nitric oxide (NO) production. These results provide a basis for studying the relationship between the structure and biological activity of arabinogalactans.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 200
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 30177181
- Full Text :
- https://doi.org/10.1016/j.carbpol.2018.08.027