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Atrial peptides inhibit oxygen consumption in kidney medullary collecting duct cells.
- Source :
-
The American journal of physiology [Am J Physiol] 1986 Aug; Vol. 251 (2 Pt 2), pp. F379-83. - Publication Year :
- 1986
-
Abstract
- Atrial natriuretic peptides (ANP) stimulate renal Na+ excretion by poorly understood mechanisms, perhaps involving direct inhibition of Na+ transport in the kidney medulla. To examine the effects of ANP on renal cells directly, we prepared highly purified cell suspensions derived from inner and outer medullary collecting duct and thick ascending limb of rabbit kidney and monitored ouabain-sensitive oxygen consumption (QO2). Human ANP diminished QO2 by 27.4 +/- 1.6% (mean +/- SE) in inner medullary collecting duct cells but had no effect in cells derived from outer medullary collecting duct or thick ascending limb. The inhibitory effect of ANP was not additive with either amiloride or ouabain. ANP was without effect in the presence of amphotericin. These results indicate that ANP inhibited Na+ entry in inner medullary collecting duct cells. ANP-mediated inhibition of QO2 was dose dependent (Ki = 5.5 X 10(-10) M) and exhibited selectivity for peptide structure. These results suggest that atrial peptides enhance renal sodium excretion partly by direct inhibition of medullary collecting duct sodium transport.
- Subjects :
- Amiloride pharmacology
Amphotericin B pharmacology
Animals
Dose-Response Relationship, Drug
Drug Interactions
Furosemide pharmacology
Humans
Kidney Medulla
Kidney Tubules, Collecting cytology
Kidney Tubules, Collecting enzymology
Osmolar Concentration
Rabbits
Sodium-Potassium-Exchanging ATPase metabolism
Atrial Natriuretic Factor pharmacology
Kidney Tubules metabolism
Kidney Tubules, Collecting metabolism
Oxygen Consumption drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9513
- Volume :
- 251
- Issue :
- 2 Pt 2
- Database :
- MEDLINE
- Journal :
- The American journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 3017127
- Full Text :
- https://doi.org/10.1152/ajprenal.1986.251.2.F379