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TBC1d24-ephrinB2 interaction regulates contact inhibition of locomotion in neural crest cell migration.
- Source :
-
Nature communications [Nat Commun] 2018 Aug 28; Vol. 9 (1), pp. 3491. Date of Electronic Publication: 2018 Aug 28. - Publication Year :
- 2018
-
Abstract
- Although Eph-ephrin signalling has been implicated in the migration of cranial neural crest (CNC) cells, it is still unclear how ephrinB transduces signals regulating this event. We provide evidence that TBC1d24, a putative Rab35-GTPase activating protein (Rab35 GAP), complexes with ephrinB2 via the scaffold Dishevelled (Dsh) and mediates a signal affecting contact inhibition of locomotion (CIL) in CNC cells. Moreover, we found that, in migrating CNC, the interaction between ephrinB2 and TBC1d24 negatively regulates E-cadherin recycling in these cells via Rab35. Upon engagement of the cognate Eph receptor, ephrinB2 is tyrosine phosphorylated, which disrupts the ephrinB2/Dsh/TBC1d24 complex. The dissolution of this complex leads to increasing E-cadherin levels at the plasma membrane, resulting in loss of CIL and disrupted CNC migration. Our results indicate that TBC1d24 is a critical player in ephrinB2 control of CNC cell migration via CIL.
- Subjects :
- Animals
Blotting, Western
Carrier Proteins genetics
Cell Movement genetics
Cell Movement physiology
Ephrin-B2 genetics
GTPase-Activating Proteins
Immunoprecipitation
Locomotion genetics
Locomotion physiology
Membrane Proteins
Microscopy, Fluorescence
Nerve Tissue Proteins
Phosphorylation
Protein Binding
Xenopus
Carrier Proteins metabolism
Embryo, Nonmammalian cytology
Embryo, Nonmammalian metabolism
Ephrin-B2 metabolism
Neural Crest cytology
Neural Crest metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 30154457
- Full Text :
- https://doi.org/10.1038/s41467-018-05924-9