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Flaxseed Oil Attenuates Hepatic Steatosis and Insulin Resistance in Mice by Rescuing the Adaption to ER Stress.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2018 Oct 17; Vol. 66 (41), pp. 10729-10740. Date of Electronic Publication: 2018 Sep 13. - Publication Year :
- 2018
-
Abstract
- Increasing evidence has demonstrated the benefits of α-linolenic acid-rich flaxseed oil (ALA-FO) against lipid metabolism abnormality in both rodent models and humans. However, the metabolic response of FO to insulin resistance and type 2 diabetes is still inconsistent. This study aimed to explore the effect of FO on chronic high fat diet (HFD)-induced hepatic steatosis, insulin resistance, and inflammation, mainly focusing on hepatic n-3 fatty acid remodeling and endoplasmic reticulum (ER) unfolded protein response. The results showed that lard-based HFD feeding for 16 weeks (60% fat-derived calories) induced whole-body insulin resistance, lipid profile abnormality, and inflammation in mice, which was alleviated by FO in a dose-dependent manner. Moreover, FO effectively improved hepatic steatosis and insulin resistance in mice by modulating the specific location of ALA and its long-chain n-3 fatty acids across hepatic lipid fractions and enhancing insulin-stimulated phosphorylation of hepatic insulin receptor subtract-1 (IRS-1) tyrosine 632 and protein kinase B (AKT) ( p < 0.05). Importantly, the differential depositions of ALA and its long-chain n-3 fatty acids in plasma and ER membranes were observed, concomitant with the rescued ER unfolded protein response and Jun N-terminal kinase (JNK) signaling in mice liver.
- Subjects :
- Animals
Diabetes Mellitus, Type 2 metabolism
Diet, High-Fat adverse effects
Fatty Acids metabolism
Humans
Inflammation drug therapy
Insulin Receptor Substrate Proteins metabolism
Linseed Oil therapeutic use
Lipid Metabolism drug effects
Liver metabolism
MAP Kinase Kinase 4 metabolism
Male
Mice
Mice, Inbred C57BL
Proto-Oncogene Proteins c-akt metabolism
Signal Transduction drug effects
Unfolded Protein Response drug effects
alpha-Linolenic Acid therapeutic use
Endoplasmic Reticulum Stress drug effects
Fatty Liver drug therapy
Insulin Resistance physiology
Linseed Oil chemistry
alpha-Linolenic Acid chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 66
- Issue :
- 41
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 30145885
- Full Text :
- https://doi.org/10.1021/acs.jafc.8b03906