Back to Search
Start Over
Equine chorionic gonadotrophin improved vascularization of feline ovarian tissue xenografted into immunosuppressed mice.
- Source :
-
Theriogenology [Theriogenology] 2018 Nov; Vol. 121, pp. 78-81. Date of Electronic Publication: 2018 Aug 14. - Publication Year :
- 2018
-
Abstract
- The objective of this study was to evaluate effects of eCG on vascularization and development of feline ovarian tissue xenografted to immunosuppressed mice. Feline ovarian fragments (∼1 mm <superscript>3</superscript> ) were transplanted under the renal capsule of 20 adult, ovariectomized, C57BL/6 SCID female mice. At 45 d after transplantation, 10 mice (controls) were euthanized and the remainder given 10 IU of eCG (and sacrificed 48 h later). Transplants were recovered immediately after death, fixed, sectioned, and stained with periodic acid-Schiff (PAS). Fragment volume (Cavallieri principle) and vascularization were assessed. Mean xenotransplant volume for control and treatment groups was 0.17 ± 0.03 and 0.37 ± 0.13 mm <superscript>3</superscript> , respectively (P = 0.0952); vascular volume density, 30.3 ± 11.3 and 49.1 ± 8.9% (P = 0.0281); surface density, 4.1 ± 2.4 and 6.2 ± 1.7 μm <superscript>-1</superscript> (P = 0.2222); and vessel total surface, 0.63 ± 0.24 μm <superscript>2</superscript> and 2.28 ± 1.05 μm <superscript>2</superscript> (P = 0.0079). In conclusion, eCG significantly increased vascular volume density of xenotransplanted ovarian tissue and improved its development.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Female
Gonadotropins, Equine pharmacology
Mice, Inbred C57BL
Mice, SCID
Ovary blood supply
Tissue Transplantation methods
Transplantation, Heterologous
Cats
Chorionic Gonadotropin therapeutic use
Heterografts blood supply
Neovascularization, Physiologic drug effects
Ovary transplantation
Tissue Transplantation veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3231
- Volume :
- 121
- Database :
- MEDLINE
- Journal :
- Theriogenology
- Publication Type :
- Academic Journal
- Accession number :
- 30144734
- Full Text :
- https://doi.org/10.1016/j.theriogenology.2018.08.006