Back to Search
Start Over
Multivariate optimization of a method for the determination of fatty acids in dental biofilm by GC-MS.
- Source :
-
Bioanalysis [Bioanalysis] 2018 Aug 01; Vol. 10 (16), pp. 1319-1333. Date of Electronic Publication: 2018 Aug 24. - Publication Year :
- 2018
-
Abstract
- Aim: Phospholipid fatty acid methyl ester (FAME) analysis offers a simple option additionally to 16S rRNA sequencing to characterize microbial communities and to monitor changes. A method was established for the characterization of dental plaque via FAME profiles.<br />Methodology: Fatty acids were determined as FAMEs (direct, acidic transesterification) and analyzed by GC-MS using an optimized temperature gradient. The transesterification reaction was optimized using a fractional factorial central composite face-centered design.<br />Results: Optimal conditions for the transesterification in methanol/toluene: hydrochloric acid concentration 2% (w/v), reaction time 40 min, temperature 110 °C. Method validation showed satisfactory accuracy, precision and linearity.<br />Conclusion: The method provides a useful tool to characterize plaque via FAME profiles and was successfully applied to samples from ten subjects demonstrating its applicability.
Details
- Language :
- English
- ISSN :
- 1757-6199
- Volume :
- 10
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Bioanalysis
- Publication Type :
- Academic Journal
- Accession number :
- 30141343
- Full Text :
- https://doi.org/10.4155/bio-2018-0106