Back to Search
Start Over
Production of idiotypic and anti-idiotypic antibodies by BALB/c mice in response to immunizations with glucagon, vasopressin, or insulin: supporting evidence for the network concept.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1986 Jul 15; Vol. 137 (2), pp. 597-602. - Publication Year :
- 1986
-
Abstract
- After immunizations with glucagon or vasopressin, either conjugated to keyhole limpet hemocyanin or adsorbed to polyvinylpyrrolidone, both anti-hormone and anti-receptor activities were detectable in the serum of injected mice. Anti-hormone activity was identified by ELISA techniques; anti-receptor activity, by determining the ability of serum samples to compete with labeled hormone for glucagon or vasopressin receptors on rat liver plasma membranes. Anti-receptor activity appeared only after the peak anti-hormone response to each immunogen had been established, and required intensive immunizations (six to nine monthly injections). The presence of anti-idiotypic antibodies in serum samples containing glucagon or vasopressin anti-receptor activity was confirmed by demonstrating selective binding of such samples to corresponding rabbit idiotypic antibodies. Serum from mice immunized with insulin also contained anti-hormone activity, as determined by ELISA, and anti-receptor activity, as determined by noting insulin-mimicking properties in stimulating glucose transport in rat adipocytes. The anti-insulin receptor activity developed after only one boost with the hormone. These results are consistent with Jerne's network hypothesis in that the glucagon, vasopressin, and insulin anti-receptor activity may be attributed to antibodies produced in mice as part of an idiotypic-anti-idiotypic network.
- Subjects :
- Animals
Female
Glucagon metabolism
Immunization
Insulin metabolism
Insulin Antibodies biosynthesis
Mice
Mice, Inbred BALB C
Receptor, Insulin immunology
Receptors, Angiotensin immunology
Receptors, Glucagon
Receptors, Vasopressin
Vasopressins metabolism
Antibodies, Anti-Idiotypic biosynthesis
Glucagon immunology
Immunoglobulin Idiotypes immunology
Insulin immunology
Receptors, Cell Surface immunology
Vasopressins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 137
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 3013996