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Aeromonas veronii and aerolysin are important for the pathogenesis of motile aeromonad septicemia in cyprinid fish.
- Source :
-
Environmental microbiology [Environ Microbiol] 2018 Sep; Vol. 20 (9), pp. 3442-3456. Date of Electronic Publication: 2018 Sep 20. - Publication Year :
- 2018
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Abstract
- Aeromonas species are ubiquitous inhabitants of freshwater environments, and are responsible for fish motile aeromonad septicemia (MAS). A. hydrophila is implicated as the primary etiologic agent of MAS. Here, we analysed MAS epidemiological data for cyprinid fish in southern China, and found that A. veronii infections dominated. Consistent with this observation, A. veronii isolates were generally more virulent than A. hydrophila isolates when infecting germ-free zebrafish larvae via continuous immersion challenge. Through in vivo screening of the transposon library of the A. veronii strain Hm091, aerolysin was identified as the key virulence factor. Further results indicated that A. veronii Hm091 aerolysin disrupts the intestinal barrier of zebrafish, enabling systematic invasion by not only A. veronii Hm091 in a mono-infection, but also A. hydrophila NJ-1 in a mixed infection. Moreover, the differences in aerolysin expression and activity were the major contributor to the observed differences between the A. veronii and A. hydrophila strains regarding invasion efficacy via intestine. Together, our results provide new insights into the aetiology and pathogenesis of Aeromonas infections, and highlight the importance of A. veronii-targeted treatments in future efforts against MAS.<br /> (© 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.)
- Subjects :
- Aeromonas isolation & purification
Aeromonas veronii genetics
Animals
Bacterial Toxins genetics
Bacterial Toxins toxicity
China
Gram-Negative Bacterial Infections microbiology
Pore Forming Cytotoxic Proteins genetics
Pore Forming Cytotoxic Proteins toxicity
Sepsis microbiology
Virulence
Virulence Factors genetics
Virulence Factors metabolism
Virulence Factors toxicity
Zebrafish microbiology
Aeromonas veronii metabolism
Aeromonas veronii pathogenicity
Bacterial Toxins metabolism
Fish Diseases microbiology
Gram-Negative Bacterial Infections veterinary
Pore Forming Cytotoxic Proteins metabolism
Sepsis veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1462-2920
- Volume :
- 20
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 30136361
- Full Text :
- https://doi.org/10.1111/1462-2920.14390