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Intravitreal pro-inflammatory cytokines in non-obese diabetic mice: Modelling signs of diabetic retinopathy.
- Source :
-
PloS one [PLoS One] 2018 Aug 22; Vol. 13 (8), pp. e0202156. Date of Electronic Publication: 2018 Aug 22 (Print Publication: 2018). - Publication Year :
- 2018
-
Abstract
- Diabetic retinopathy is a vascular disease of the retina characterised by hyperglycaemic and inflammatory processes. Most animal models of diabetic retinopathy are hyperglycaemia-only models that do not account for the significant role that inflammation plays in the development of the disease. In the present study, we present data on the establishment of a new animal model of diabetic retinopathy that incorporates both hyperglycaemia and inflammation. We hypothesized that inflammation may trigger and worsen the development of diabetic retinopathy in a hyperglycaemic environment. Pro-inflammatory cytokines, IL-1β and TNF-α, were therefore injected into the vitreous of non-obese diabetic (NOD) mice. CD1 mice were used as same genetic background controls. Fundus and optical coherence tomography images were obtained before (day 0) as well as on days 2 and 7 after intravitreal cytokine injection to assess vessel dilation and beading, retinal and vitreous hyper-reflective foci and retinal thickness. Astrogliosis and microgliosis were assessed using immunohistochemistry. Results showed that intravitreal cytokines induced vessel dilation, beading, severe vitreous hyper-reflective foci, retinal oedema, increased astrogliosis and microglia upregulation in diabetic NOD mice. Intravitreal injection of inflammatory cytokines into the eyes of diabetic mice therefore appears to provide a new model of diabetic retinopathy that could be used for the study of disease progression and treatment strategies.<br />Competing Interests: We, the authors declare that no competing interests exist.
- Subjects :
- Animals
Biomarkers
Blood Glucose
Cytokines administration & dosage
Diabetes Mellitus, Experimental
Diabetic Retinopathy diagnosis
Disease Models, Animal
Female
Immunohistochemistry
Inflammation Mediators administration & dosage
Intravitreal Injections
Mice
Mice, Inbred NOD
Models, Biological
Severity of Illness Index
Tomography, Optical Coherence
Cytokines metabolism
Diabetic Retinopathy etiology
Diabetic Retinopathy metabolism
Inflammation Mediators metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 13
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 30133488
- Full Text :
- https://doi.org/10.1371/journal.pone.0202156