Polyphosphoinositides undergo charge neutralization in the physiological pH range: a 31P-NMR study.

The charge state of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate was determined as a function of pH by way of 31P-NMR spectroscopy. The pK values for the first protonation of the phosphomonoester residues in phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate were found to be 6.2 and 6.6, respectively, for the 4-phosphate moiety, and 7.7 for the 5-phosphate moiety.