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Dynamic changes in miR-124 levels in patients with acute cerebral infarction.

Authors :
Sun M
Hou X
Ren G
Zhang Y
Cheng H
Source :
The International journal of neuroscience [Int J Neurosci] 2019 Jul; Vol. 129 (7), pp. 649-653. Date of Electronic Publication: 2019 Feb 20.
Publication Year :
2019

Abstract

Objective: To investigate the changes in serum miR-124 levels in patients with acute cerebral infarction (ACI) and elucidate the underlying mechanism by a dynamic monitor.<br />Methods: Fifty-four patients with ACI and 51 healthy controls were included in our study. Baseline characteristics and blood samples were collected for further analysis. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the serum miR-124 levels. The dual-luciferase reporter assay was used to evaluate the effect of miR-124 on iASPP, a protein that inhibits apoptosis stimulating proteins in the p53 family.<br />Results: Compared with normal controls, the miR-124 levels in the ACI group rapidly decreased at phase 1 (within 24 h after ischemia) (p < 0.001) and then gradually increased at phase 2 (48 ∼ 72 h after ischemia) (p < 0.001) and phase 3 (the 7th day after ischemia) (p < 0.001). The dual-luciferase reporter assay showed that miR-124 down-regulates iASPP expression in 293T cells.<br />Conclusion: The miR-124 levels are down-regulated in ACI patients. The dynamic changes of miR-124 might provide a possible method for the detection of ischemic stroke. Highlights The difference in miR-124 expression levels between ACI patients and normal controls. Dynamic changes of miR-124 expression levels in ACI patients. The down-regulation of miR-124 upon iASPP expression.

Details

Language :
English
ISSN :
1563-5279
Volume :
129
Issue :
7
Database :
MEDLINE
Journal :
The International journal of neuroscience
Publication Type :
Academic Journal
Accession number :
30124350
Full Text :
https://doi.org/10.1080/00207454.2018.1513931