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Inhalable liposomes of Glycyrrhiza glabra extract for use in tuberculosis: formulation, in vitro characterization, in vivo lung deposition, and in vivo pharmacodynamic studies.
- Source :
-
Drug development and industrial pharmacy [Drug Dev Ind Pharm] 2019 Jan; Vol. 45 (1), pp. 11-20. Date of Electronic Publication: 2018 Sep 07. - Publication Year :
- 2019
-
Abstract
- Objective: The current study involves the development of liposomal dry powder for inhalation (LDPI) containing licorice extract (LE) for use in tuberculosis.<br />Significance: The current epidemiology of tuberculosis along with the increasing emergence of resistant forms of tuberculosis necessitates the need for developing alternative efficacious medicines for treatment. Licorice is a medicinal herb with reported activity against Mycobacterium tuberculosis.<br />Methods: Liposomes with LE were prepared by thin film hydration technique and freeze dried to obtain LDPI. The comprehensive in vitro and in vivo characterization of the LDPI formulation was carried out.<br />Results: The particle size of liposomes was around 210 nm with drug entrapment of almost 75%. Transmission electron microscopy revealed spherical shape of liposome vesicles. The flow properties of the LDPI were within acceptable limits. Anderson Cascade Impactor studies showed the mean median aerodynamic diameter, geometric standard deviation and fine particle fraction of the LDPI to be 4.29 µm, 1.23, and 54.68%, respectively. In vivo lung deposition studies of LDPI in mice showed that almost 46% of the drug administered reaches the lungs and 16% of administered drug is retained in the lungs after 24 hours of administration. The in vivo pharmacodynamic evaluation of the LDPI showed significant reduction in bacterial counts in lungs as well as spleen of TB-infected mice.<br />Conclusions: LE LDPI thus has a promising potential to be explored as an effective anti-tubercular medicine or as an adjunct to existing anti-tubercular drugs.
- Subjects :
- Administration, Inhalation
Animals
Antitubercular Agents chemistry
Antitubercular Agents pharmacokinetics
Drug Compounding
Liposomes
Lung metabolism
Mice
Plant Extracts chemistry
Plant Extracts isolation & purification
Plant Extracts pharmacokinetics
Plant Roots
Tuberculosis metabolism
Antitubercular Agents administration & dosage
Glycyrrhiza
Lung drug effects
Particle Size
Plant Extracts administration & dosage
Tuberculosis drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5762
- Volume :
- 45
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Drug development and industrial pharmacy
- Publication Type :
- Academic Journal
- Accession number :
- 30122088
- Full Text :
- https://doi.org/10.1080/03639045.2018.1513025