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A rapid and reliable assay to determine flumequine, marbofloxacin, difloxacin, and sarafloxacin in commonly consumed meat by micellar liquid chromatography.
- Source :
-
Journal of the science of food and agriculture [J Sci Food Agric] 2019 Feb; Vol. 99 (3), pp. 1375-1383. Date of Electronic Publication: 2018 Oct 08. - Publication Year :
- 2019
-
Abstract
- Background: Micellar liquid chromatography - fluorescence detection was used to determine the antibiotics flumequine, marbofloxacin, difloxacin, and sarafloxacin in porcine, bovine, poultry, ovine, caprine, rabbit, and equine meat, to verify compliance with EU Regulation 37/2010 with regard to the occurrence of veterinary drugs in food.<br />Results: The analytes were isolated from the matrix by ultrasonication-assisted leaching in a micellar solution, and the supernatant was filtered and directly injected. The fluoroquinolones were resolved in < 19 min using a C18 column, with an isocratic mobile phase of 0.05 mol L <superscript>-1</superscript> sodium dodecyl sulfate - 8% 1-butanol - 0.5% triethylamine buffered at pH 3. The limits of quantification (0.01-0.05 mg kg <superscript>-1</superscript> ) were below the maximum residue limits (0.15-0.4 mg kg <superscript>-1</superscript> ). The method was validated by EU Commission Decision 2002/657/EC guidelines.<br />Conclusion: The method shows practical advantages such as simplicity, low cost, eco-friendliness, safety, and applicability for routine analysis, and is useful for surveillance programs. © 2018 Society of Chemical Industry.<br /> (© 2018 Society of Chemical Industry.)
Details
- Language :
- English
- ISSN :
- 1097-0010
- Volume :
- 99
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of the science of food and agriculture
- Publication Type :
- Academic Journal
- Accession number :
- 30120781
- Full Text :
- https://doi.org/10.1002/jsfa.9314