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Ultrastructural, Molecular and Functional Mapping of GABAergic Synapses on Dendritic Spines and Shafts of Neocortical Pyramidal Neurons.
- Source :
-
Cerebral cortex (New York, N.Y. : 1991) [Cereb Cortex] 2019 Jul 05; Vol. 29 (7), pp. 2771-2781. - Publication Year :
- 2019
-
Abstract
- The location of GABAergic synapses on dendrites is likely key for neuronal integration. In particular, inhibitory inputs on dendritic spines could serve to selectively veto or modulate individual excitatory inputs, greatly expanding the computational power of individual neurons. To investigate this, we have undertaken a combined functional, molecular, and ultrastructural mapping of the location of GABAergic inputs onto dendrites of pyramidal neurons from upper layers of juvenile mouse somatosensory cortex. Using two-photon uncaging of GABA, intracellular labeling with gerphyrin intrabodies, and focused ion beam milling with scanning electron microscopy, we find that most (96-98%) spines lack GABAergic synapses, although they still display GABAergic responses, potentially due to extrasynaptic GABA receptors. We conclude that GABAergic inputs, in practice, contact dendritic shafts and likely control clusters of excitatory inputs, defining functional zones on dendrites.<br /> (© The Author(s) 2018. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.)
- Subjects :
- Animals
Dendritic Spines physiology
GABAergic Neurons physiology
Mice
Pyramidal Cells physiology
Pyramidal Cells ultrastructure
Somatosensory Cortex physiology
Synapses physiology
Dendritic Spines ultrastructure
GABAergic Neurons ultrastructure
Somatosensory Cortex ultrastructure
Synapses ultrastructure
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2199
- Volume :
- 29
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cerebral cortex (New York, N.Y. : 1991)
- Publication Type :
- Academic Journal
- Accession number :
- 30113619
- Full Text :
- https://doi.org/10.1093/cercor/bhy143