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Purification and Identification of Astaxanthin and Its Novel Derivative Produced by Radio-tolerant Sphingomonas astaxanthinifaciens.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1852, pp. 171-192. - Publication Year :
- 2018
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Abstract
- The red diketocarotenoid, astaxanthin, exhibits extraordinary health-promoting activities such as antioxidant, anti-inflammatory, antitumor, and immune booster, which may potentially protect against many degenerative diseases such as cancers, heart diseases, and exercise-induced fatigue. These numerous health benefits and consumer interest in natural products have therefore increased the market demand of astaxanthin as a nutraceutical and medicinal ingredient in food, aquaculture feed, and pharmaceutical industries. Consequently, many research efforts have been made to discover novel microbial sources with effective biotechnological production of astaxanthin. Using a rapid screening method based on 16S rRNA gene, and effective HPLC-Diode array-MS methods for carotenoids analysis, we isolated a novel astaxanthin-producing bacterium (strain TDMA-17 <superscript>T</superscript> ) that belongs to the family Sphingomonadaceae (Asker et al., FEMS Microbiol Lett 273:140-148, 2007).In this chapter, we provide a comprehensive description of the methods used for the analysis and identification of carotenoids produced by strain TDMA-17 <superscript>T</superscript> . We will also describe the methods of isolation and identification for a novel bacterial carotenoid (an astaxanthin derivative), a major carotenoid that is produced by the novel strain. Finally, the identification methods of the novel strain will be summarized.
- Subjects :
- Antioxidants chemistry
Antioxidants isolation & purification
Antioxidants metabolism
Chromatography, High Pressure Liquid
Molecular Structure
Phylogeny
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Spectrum Analysis
Sphingomonas chemistry
Sphingomonas ultrastructure
Xanthophylls biosynthesis
Xanthophylls chemistry
Xanthophylls isolation & purification
Sphingomonas metabolism
Sphingomonas radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1852
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 30109631
- Full Text :
- https://doi.org/10.1007/978-1-4939-8742-9_10