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Difference in molecular size of receptors for alpha-rat atrial natriuretic polypeptide among the kidney, aorta, and adrenal gland as identified by direct UV-photoaffinity labeling.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1986 Apr 14; Vol. 136 (1), pp. 200-7. - Publication Year :
- 1986
-
Abstract
- In order to identify the molecular size of receptors for alpha-rat atrial natriuretic polypeptide (alpha-rANP), we utilized the direct UV irradiation method for photoaffinity labeling with the biologically active [125I] alpha-rANP. In the preparation of isolated glomerulus and the inner medullary collecting duct (IMCT)-rich fraction, the autoradiograms of the electrophoresed sodium dodecyl sulfate (SDS)-polyacrylamide gels showed a single radioactive band which is displaceable with unlabeled alpha-rANP. The dose-dependent displacement fit very well with a binding-inhibition curve representing the binding affinity of 6.5 X 10(-10) M. The molecular size of the ligand-receptor complex was about 65,000 daltons for both glomerulus and IMCT-rich fraction. In contrast, in homogenate of the aorta and adrenal gland, the ligand-receptor complex was 140,000 daltons.
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 136
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 3010963
- Full Text :
- https://doi.org/10.1016/0006-291x(86)90895-8