Automated radiosynthesis of [ 18 F]FBEM, a sulfhydryl site specific labeling agent for peptides and proteins.

Authors :: Lim K
Ropchan J
Kiesewetter DO
Chen X
Huang Y
Source :: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine [Appl Radiat Isot] 2018 Oct; Vol. 140, pp. 294-299. Date of Electronic Publication: 2018 Jul 29.
Publication Year :: 2018
Abstract: In the process of developing [ <superscript>18</superscript> F]FBEM coupled target peptide, we have instituted a robust automated synthesis of [ <superscript>18</superscript> F]FBEM, a sulfhydryl (-SH) site specific agent for radiolabeling of peptides and proteins. The radiosynthesis generated 1.67-3.89 GBq (45.1-105.1 mCi, 7.5-18.8% non-decay corrected yield) of [ <superscript>18</superscript> F]FBEM from 22.2 GBq (600 mCi) of starting [ <superscript>18</superscript> F]fluoride with molar activity of 31.8 ± 5.3 GBq/µmol (0.86 ± 0.14 mCi/nmol) (n = 3) at the end of synthesis. Radiochemical purity was greater than 98%, and total synthesis time was ~90 min.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Language :: English
ISSN :: 1872-9800
Volume :: 140
Database :: MEDLINE
Journal :: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine
Publication Type :: Academic Journal
Accession number :: 30098587
Full Text :: https://doi.org/10.1016/j.apradiso.2018.07.033

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