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Different intestinal tropism of the G2b Taiwan porcine epidemic diarrhea virus-Pintung 52 strain in conventional 7-day-old piglets.

Authors :
Peng JY
Chang CY
Kao CF
Chang YC
Hsueh CS
Jeng CR
Cheng IC
Pang VF
Tsai PS
Chang HW
Source :
Veterinary journal (London, England : 1997) [Vet J] 2018 Jul; Vol. 237, pp. 69-75. Date of Electronic Publication: 2018 Jun 07.
Publication Year :
2018

Abstract

The group 2b (G2b) porcine epidemic diarrhea virus (PEDV) that emerged in 2013 has since caused devastating diseases and economic loss. The full-length genome of the G2b Taiwan PEDV-Pintung 52 (PEDV-PT) strain and its intestinal tropism by evaluating the pathological changes in the original PEDV-PT infected field piglet and orally inoculation of either 10, 10 <superscript>3</superscript> , or 10 <superscript>5</superscript> 50% tissue culture infective dose/mL (TCID <subscript>50</subscript> /mL) of the plaque-purified PEDV-PT-Passage 5 (P5) in 7-day-old conventional piglets were analyzed. Phylogenetic analysis of the full-length genome indicated that the G2b Taiwan PEDV-PT strain was closely related to the North American G2b PEDV strains. Some pathological features of the G2b Taiwan PEDV-PT infection, including the absence of lesions and antigen signal in the crypt epithelial cells of the jejunum and ileum and in the villus enterocytes of the duodenum and colon, were different from those of infections by the North American G2b PEDV strains. This difference in the intestinal tropism of the G2b Taiwan PEDV-PT strain highlights the importance of studying the pathogenicities of different PEDV variants. Moreover, similar distributions of PEDV antigens and lesions in the G2b Taiwan PEDV-PT infected field piglet and its plaque-purified isolate, PEDV-PT-P5, inoculated piglets indicating that the plaque-purified PEDV-PT-P5 viral stock could facilitate the preclinical evaluation of vaccines and other interventions aimed at preventing the G2b PEDV infection.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Details

Language :
English
ISSN :
1532-2971
Volume :
237
Database :
MEDLINE
Journal :
Veterinary journal (London, England : 1997)
Publication Type :
Academic Journal
Accession number :
30089548
Full Text :
https://doi.org/10.1016/j.tvjl.2018.06.002