Back to Search
Start Over
Assessing hepatitis B immunity using dried blood spot samples from HIV+ individuals.
- Source :
-
Journal of medical virology [J Med Virol] 2018 Dec; Vol. 90 (12), pp. 1863-1867. Date of Electronic Publication: 2018 Sep 03. - Publication Year :
- 2018
-
Abstract
- This study aims to evaluate the utility of an optimized enzyme immunoassay (EIA) to detect and quantify antibodies against hepatitis B surface antibody (anti-HBs) in dried blood spots (DBSs) within the context of human immunodeficiency virus (HIV) status. Serum and DBS samples were obtained from 56 HIV+ and 99 HIV- patients and subjected to EIA for the detection of anti-HBs, where sample volume and cut off value were modified for DBS testing. Sensitivities of anti-HBs detection in DBS were 79.8% and 76.8% in HIV- and HIV+ subjects, respectively. Concordant results for anti-HBs in serum and DBS presented high mean CD8 cell counts, HIV viral load and optical density (OD) values of anti-HBs. Anti-HBs titers were significantly higher in serum, whether or not anti-HBs titers were detected in DBS. It was possible to detect anti-HBs in DBS as low as 17.4 and 27.3 IU/mL among HIV+ and HIV- subjects, respectively. In conclusion, DBS can be used to detect and quantify anti-HBs in HIV-infected individuals, which could increase access to diagnosis and vaccination.<br /> (© 2018 Wiley Periodicals, Inc.)
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Female
Hepatitis B Surface Antigens immunology
Humans
Immunoenzyme Techniques methods
Male
Middle Aged
Sensitivity and Specificity
Young Adult
Desiccation
HIV Infections
Hepatitis B immunology
Hepatitis B Antibodies blood
Hepatitis B virus immunology
Mass Screening methods
Specimen Handling methods
Subjects
Details
- Language :
- English
- ISSN :
- 1096-9071
- Volume :
- 90
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of medical virology
- Publication Type :
- Academic Journal
- Accession number :
- 30085359
- Full Text :
- https://doi.org/10.1002/jmv.25275